Amaç
Preimplantation genetic diagnosis (PGD) of aneuploidy was performed on embryos of 108 patients undergoing IVF with identitication of either advanced maternal age, repeated implantation failures in IVF/ICSI or poor obstetric history usually in the form of recurrent abortion of no demonstrable cause or known genetic abnormality between April 2001 and June 2002.
Yöntem
For 108 ICSI/PGD/ET cycles, 586-day three embryos were biopsied and one blastomere from each was fixed for aneuploidy screening. MultiVysion PB (Vysis) hybridization kit was used for detection of chromosomes 13, 16, 18, 21, 22 and X, Y. Day five embryo transfer was performed for the embryos evaluated as normal.
Bulgular
Of the 586 embryos 372 (65%) were found aneuploid and the remaining 205 (35%) embryos were evaluated as euploid, resulting in day five blastocyst transfer. The aneuploidy rates for ehromosomes 13. 16, 18, 21, 22 and XY were as follows; 18%, 13%, 20%, 19%, 10%, 19%. A total of 141 embryos were transferred to 83 (77%) patients (mean 1.2; min: 1, max: 4). We achieved a positive pregnaney test on day 10 in 21 patients giving us a 19.4% pregnaney rate per cycle. Our clinical pregnaney rate per cycle and implantation rate per replaced embryo was 16.6% (18/108) and 12.7%; (18/141) respeetively. The ongoing pregnaney rate per PGD cycle was 11.9 (18/108) while five babies were delivered without any perinatal complications. All ongoing pregnancies were confirmed by prenatal diagnosis informing us that there was no misdiagnosis including the performed aneuploidy screening.
Sonuç
An inereased pregnancy rate was achieved by PGD of aneuploidy in poor prognosis IVF patients and also this technique may be recommended to overcome the complications of multiple pregnancies of IVF treatment by selecting the euploid and good quality embryos, which enables to decrease the number of transferred embryos.
Anahtar Kelimeler
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